How can the reliability of the AccordQuick system be guaranteed in detecting multiple pathogens?
Publish Time: 2025-05-27
In the field of modern molecular diagnosis, rapid and accurate identification of multiple pathogens is essential for clinical diagnosis, public health monitoring and epidemic response. The AccordQuick series of fully automatic thin-film microfluidic nucleic acid detection all-in-one machines are advanced detection platforms designed to meet this demand. It integrates thin-film microfluidic technology and fluorescent quantitative PCR technology to achieve one-stop operation from nucleic acid extraction to amplification analysis. While ensuring efficiency and convenience, it also fully guarantees the high sensitivity, strong specificity and repeatability of results for the detection of multiple pathogens.
The core of the AccordQuick system lies in its use of thin-film microfluidic chip technology, which highly integrates the complex nucleic acid extraction, purification, amplification and other steps in traditional laboratories into a miniaturized microfluidic chip. This closed automated process not only reduces human operation errors, but also effectively avoids the risk of cross-contamination between samples.
At the same time, the system is equipped with mature fluorescent quantitative PCR (qPCR) technology, which is currently the "gold standard" for pathogen nucleic acid detection. This technology can achieve qualitative and quantitative analysis of the nucleic acid of the target pathogen by real-time monitoring of the changes in the fluorescent signal during the amplification process, with extremely high sensitivity and accuracy.
2. Parallel detection of multiple targets to improve the comprehensiveness of diagnosis
The AccordQuick system supports multi-channel detection design, which can detect multiple pathogens or gene targets in the same reaction system at the same time. For example, for respiratory infections, the system can detect multiple common pathogens such as influenza virus A/B, adenovirus, rhinovirus, and Mycoplasma pneumoniae at one time, greatly improving the detection efficiency and diagnostic coverage.
In addition, each detection channel undergoes strict primer and probe screening to ensure high specificity and avoid false detection or missed detection due to sequence similarity, thereby ensuring the reliability of the results.
3. Standardized process reduces human interference and improves repeatability
Traditional nucleic acid testing relies on manual operation of multiple links, which is prone to introduce errors. The AccordQuick system realizes the automation of the entire process from nucleic acid extraction, PCR amplification to data analysis. All steps are run according to the preset program without manual intervention, which significantly reduces the differences between experiments or operators and improves the consistency and repeatability of the test results.
4. Built-in quality control mechanism to ensure data credibility
In order to further improve the reliability of detection, the AccordQuick system is equipped with a complete internal standard quality control system, including positive control, negative control and endogenous internal standard, which is used to monitor whether the entire detection process is normal and prevent the occurrence of false negatives or false positives.
At the same time, the system has an automatic interpretation function. Combined with the supporting software, it can generate clear and intuitive result reports and support the export of original data for secondary verification, which enhances the traceability and scientificity of the results.
5. Wide applicability verification, suitable for various scenarios
The AccordQuick system has been verified in multiple clinical and field testing environments, covering multiple application scenarios such as hospitals, CDCs, and mobile testing vehicles. Whether it is the detection of viruses, bacteria or fungal pathogens, it has shown excellent performance, especially in the response to sudden infectious diseases such as the COVID-19 epidemic, showing its outstanding capabilities in rapid response, accurate detection, and batch screening.
In summary, the AccordQuick system has built an efficient, accurate and stable pathogen detection platform through advanced thin-film microfluidic chip technology, fluorescent quantitative PCR technology, multiple target detection capabilities, fully automated processes, built-in quality control mechanisms and wide applicability. It not only meets the high standards of clinical and public health fields, but also provides a solid technical foundation for future multi-inspection and intelligent detection.
