How does AccordEdge aystem-quadruple run achieve a fully automated process from sample to result?
Publish Time: 2025-05-19
AccordEdge aystem-quadruple run achieves a fully automated process from sample to result, mainly relying on its advanced design and fully enclosed thin film microfluidic bag technology.
1. Sample preparation and loading
First, users need to load biological samples (such as blood, saliva or tissue samples) into specially designed fully enclosed thin film microfluidic bags. These bags are designed to accommodate and process small amounts of samples while ensuring that the entire process is carried out in a closed environment to reduce the risk of contamination.
2. Cell lysis
Once the sample is loaded, AccordEdge aystem-quadruple run automatically performs the cell lysis step. By adding a specific lysis buffer to the microfluidic bag and using the system's built-in mechanism to apply appropriate pressure or temperature conditions, the cell membrane is ruptured and the DNA or RNA in the cell is released.
3. DNA/RNA purification
Next is the DNA/RNA purification process. Through a series of preset chemical reactions and physical operations (such as centrifugation, filtration, etc.), the system effectively separates and cleans high-quality nucleic acid materials, removes proteins, lipids and other impurities, and ensures the accuracy of subsequent experiments.
4. Template elution
After obtaining pure DNA/RNA, the system will proceed to the template elution step. During this process, the target nucleic acid will be resuspended in a buffer suitable for molecular amplification to prepare for the next step.
5. Molecular amplification
AccordEdge aystem-quadruple run supports a variety of molecular amplification technologies, including but not limited to PCR (polymerase chain reaction). The system can accurately control temperature cycles to ensure maximum amplification efficiency, thereby generating sufficient nucleic acid for detection.
6. Optical detection
Finally, the amplified nucleic acid sample will be optically detected. AccordEdge aystem-quadruple run is equipped with a highly sensitive optical sensor that can monitor fluorescence signals or other optical changes in real time to determine the presence and concentration of target molecules. All data will be automatically recorded and easy-to-understand reports will be generated.
7. Data analysis and result output
After completing all the above steps, the system automatically analyzes the collected data and gives the diagnosis results according to the preset standards. Users can view the final results through a simple interface without complex operations or professional knowledge.
Through this highly integrated design, AccordEdge aystem-quadruple run not only simplifies the molecular diagnosis process, but also improves work efficiency and the reliability of results. It is very suitable for resource-limited environments such as doctor's offices, clinics and even shrimp farms.
